Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Surface-enhanced Raman scattering (SERS) has the capability to detect single molecules for which high field enhancement is required. Single-molecule (SM) SERS able provide molecule-specific spectroscopic information about individual and therefore yields more detailed chemical than other SM detection techniques. At same time, there potential unravel from measurements that remain hidden in of bulk material. This protocol outlines using a DNA origami nanoantenna (DONA) combination with atomic force microscopy (AFM) spectroscopy. A fork structure two gold nanoparticles are combined form DONAs, 1.2-2.0 nm gap between them. allows an up 1011-fold signal enhancement, enabling molecules. The further demonstrates placement analyte molecule hot spot, process AFM imaging, subsequent overlaying imaging measure DONA.